What method is used to separate water and sugar?
The separation method that can be used to separate the components of a mixture of water and sugar would be the Evaporation method. This is used in the separation of homogeneous mixtures where there is no interest in conserving the solvent, in this case the water, since it will be lost at the time of evaporation.
What property allows separation?
In extraction, the property that is used to carry out the separation is the solubility or affinity of a substance with a solvent.
What problems can arise in the packing of a column?
The lack of uniformity in the packing of a column results in the formation of irregular zones that can cause poor separation. Columns used in liquid chromatography generally have a diameter large enough that mechanical vibration can be applied.
Why does a peak split in HPLC?
Split peaks appear due to the effect of injection solvents stronger than mobile phases, they can even cause peak broadening, it is necessary to keep the concentration of organic in sample solvent less than or equal to the mobile phase.
What detectors are used in CG?
Most used detectors in Gas Chromatography
- Thermal Conductivity Detector. It measures the thermal conductivity of the carrier gas, caused by the presence of eluted substances.
- Flame Ionization Detector.
- Electronic Capture Detector.
- Flame Photometry Detector.
- Alkaline Flame Ionization Detector.
- Mass Spectrometry Detector.
How is gas chromatography classified?
There are two types of gas chromatography: gas-solid chromatography (GSC) and gas-liquid chromatography (GLC). Gas-liquid chromatography has great application in all fields of science and its name is usually abbreviated as gas chromatography (GC).
What is the classification of Chromatographic techniques according to the physical state of the mobile phase?
Chromatographic techniques are often classified by indicating the physical state of the two phases used. According to this classification, the following expressions are used: Gas-solid chromatography (GSC) Liquid-liquid chromatography (LLC)
What are Chromatographic plates?
Chromatogram: A chromatogram is a graphical or other representation of the detector response, concentration of analyte(s) in the effluent, or other quantity used as a measure of concentration in the effluent as a function of effluent volume or time.
What are theoretical plates in HPLC?
What is a «plate» in HPLC? A theoretical plate is the hypothetical stage in which two phases of a substance (liquid phase and vapor phase) are in equilibrium. However, the resolution only increases proportionally to the square root of the number of plates.